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sihdac2  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology sihdac2
    Sihdac2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sihdac2/HDAC2+siRNA/pmc12394589-395-17-18
    Average 93 stars, based on 50 article reviews
    sihdac2 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Transfection:

    Article Title: The deacetylases HDAC1/HDAC2 control JAK2 V617F -STAT signaling through the ubiquitin ligase SIAH2.
    Article Snippet: .. The plasmids used to express the GST-SIAH2 and GST-UBCH8 fusion proteins for pulldown experiments were previously described.43,44 siRNAs and transfections Gene knockdown was performed via RNAi technology by transfecting the following SMARTpool: ON-TARGETplus siRNA: siHDAC1 (Dharmacon, L-003493-00-0005), siHDAC2 (Santa Cruz, HDAC2: sc29346), SMARTpool: ON-TARGETplus siRNA (Dharmacon, HDAC3: L003496-00-0005), siSIAH2 SMARTpool: ON-TARGETplus siRNA (Dharmacon, SIAH2: L-006561-00-0005), siUBCH8 Silencer® Select siRNA (Thermo Fisher Scientific, UBE2L6: s17680) or corresponding amounts of nontargeting control siRNA (Santa Cruz, siRNA-A: sc37007). .. All transfections were performed with the NeonTM Transfection System (Invitrogen, Carlsbad, CA, USA).

    Knockdown:

    Article Title: The deacetylases HDAC1/HDAC2 control JAK2 V617F -STAT signaling through the ubiquitin ligase SIAH2.
    Article Snippet: .. The plasmids used to express the GST-SIAH2 and GST-UBCH8 fusion proteins for pulldown experiments were previously described.43,44 siRNAs and transfections Gene knockdown was performed via RNAi technology by transfecting the following SMARTpool: ON-TARGETplus siRNA: siHDAC1 (Dharmacon, L-003493-00-0005), siHDAC2 (Santa Cruz, HDAC2: sc29346), SMARTpool: ON-TARGETplus siRNA (Dharmacon, HDAC3: L003496-00-0005), siSIAH2 SMARTpool: ON-TARGETplus siRNA (Dharmacon, SIAH2: L-006561-00-0005), siUBCH8 Silencer® Select siRNA (Thermo Fisher Scientific, UBE2L6: s17680) or corresponding amounts of nontargeting control siRNA (Santa Cruz, siRNA-A: sc37007). .. All transfections were performed with the NeonTM Transfection System (Invitrogen, Carlsbad, CA, USA).

    Article Title: The deacetylases HDAC1/HDAC2 control JAK2 V617F -STAT signaling through the ubiquitin ligase SIAH2
    Article Snippet: .. Gene knockdown was performed via RNAi technology by transfecting the following SMARTpool: ON-TARGETplus siRNA: siHDAC1 (Dharmacon, L-003493-00-0005), siHDAC2 (Santa Cruz, HDAC2: sc-29346), SMARTpool: ON-TARGETplus siRNA (Dharmacon, HDAC3: L-003496-00-0005), siSIAH2 SMARTpool: ON-TARGETplus siRNA (Dharmacon, SIAH2: L-006561-00-0005), siUBCH8 Silencer ® Select siRNA (Thermo Fisher Scientific, UBE2L6 : s17680) or corresponding amounts of nontargeting control siRNA (Santa Cruz, siRNA-A: sc-37007). .. All transfections were performed with the NeonTM Transfection System (Invitrogen, Carlsbad, CA, USA).

    Article Title: HDAC1 and HDAC2 integrate checkpoint kinase phosphorylation and cell fate through the phosphatase-2A subunit PR130
    Article Snippet: .. Knockdown of HDAC1, HDAC2, HDAC3 and PR72/PR130 in HCT116 cells was performed by transfecting 20 pmol siHDAC1 (Life Technologies, #4390824), 40 pmol siHDAC2 (Santa Cruz, sc-29346), 40 pmol siHDAC3 (Life Technologies, s16878), 20–40 pmol siPR72/PR130 (Life Technologies, s10975) or corresponding amounts of non-targeting control siRNA (Eurofins MWG Operon; Santa Cruz) with Lipofectamine ® 2000 or Lipofectamine ® RNAiMAX (manufacturer’s protocol; Invitrogen, Darmstadt, Germany). .. Knockdown of ATM and ATR was performed using 40–50 pmol SMARTpool: siGENOME siRNA (Dharmacon, ATM: M-003201-04; ATR: M-003202-05).

    Control:

    Article Title: The deacetylases HDAC1/HDAC2 control JAK2 V617F -STAT signaling through the ubiquitin ligase SIAH2.
    Article Snippet: .. The plasmids used to express the GST-SIAH2 and GST-UBCH8 fusion proteins for pulldown experiments were previously described.43,44 siRNAs and transfections Gene knockdown was performed via RNAi technology by transfecting the following SMARTpool: ON-TARGETplus siRNA: siHDAC1 (Dharmacon, L-003493-00-0005), siHDAC2 (Santa Cruz, HDAC2: sc29346), SMARTpool: ON-TARGETplus siRNA (Dharmacon, HDAC3: L003496-00-0005), siSIAH2 SMARTpool: ON-TARGETplus siRNA (Dharmacon, SIAH2: L-006561-00-0005), siUBCH8 Silencer® Select siRNA (Thermo Fisher Scientific, UBE2L6: s17680) or corresponding amounts of nontargeting control siRNA (Santa Cruz, siRNA-A: sc37007). .. All transfections were performed with the NeonTM Transfection System (Invitrogen, Carlsbad, CA, USA).

    Article Title: The deacetylases HDAC1/HDAC2 control JAK2 V617F -STAT signaling through the ubiquitin ligase SIAH2
    Article Snippet: .. Gene knockdown was performed via RNAi technology by transfecting the following SMARTpool: ON-TARGETplus siRNA: siHDAC1 (Dharmacon, L-003493-00-0005), siHDAC2 (Santa Cruz, HDAC2: sc-29346), SMARTpool: ON-TARGETplus siRNA (Dharmacon, HDAC3: L-003496-00-0005), siSIAH2 SMARTpool: ON-TARGETplus siRNA (Dharmacon, SIAH2: L-006561-00-0005), siUBCH8 Silencer ® Select siRNA (Thermo Fisher Scientific, UBE2L6 : s17680) or corresponding amounts of nontargeting control siRNA (Santa Cruz, siRNA-A: sc-37007). .. All transfections were performed with the NeonTM Transfection System (Invitrogen, Carlsbad, CA, USA).

    Article Title: HDAC1 and HDAC2 integrate checkpoint kinase phosphorylation and cell fate through the phosphatase-2A subunit PR130
    Article Snippet: .. Knockdown of HDAC1, HDAC2, HDAC3 and PR72/PR130 in HCT116 cells was performed by transfecting 20 pmol siHDAC1 (Life Technologies, #4390824), 40 pmol siHDAC2 (Santa Cruz, sc-29346), 40 pmol siHDAC3 (Life Technologies, s16878), 20–40 pmol siPR72/PR130 (Life Technologies, s10975) or corresponding amounts of non-targeting control siRNA (Eurofins MWG Operon; Santa Cruz) with Lipofectamine ® 2000 or Lipofectamine ® RNAiMAX (manufacturer’s protocol; Invitrogen, Darmstadt, Germany). .. Knockdown of ATM and ATR was performed using 40–50 pmol SMARTpool: siGENOME siRNA (Dharmacon, ATM: M-003201-04; ATR: M-003202-05).

    Negative Control:

    Article Title: Trichostatin A Inhibits Epithelial Mesenchymal Transition Induced by TGF-β1 in Airway Epithelium
    Article Snippet: The supernatant was discarded, and the cells were suspended in Neon Resuspension buffer (Invitrogen) at a concentration of 6 × 10 5 cells/mL. .. Universal negative control siRNA (siControl; Santa Cruz) and small interference oligonucleotide RNA directed against siHDAC2 and siHDAC4 (Santa Cruz) were used as controls. .. Neon Electrolytic buffer (Invitrogen) was added into the Neon transfection tubes, and the tubes were then placed in the Neon transfection system device (Invitrogen) that was set to 1400 V and 30 pulses.



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    VPA regulated miRNA expression through increased histone acetylation. (A) VPA treatment increased the global protein acetylation of LX2 cells as detected by western blot using an antibody against pan acetyl-lysine, the band for histone was about 15KD. (B) The transfection condition for LX2 cells was optimized by Cy3 labeled siRNA transfection control (Cy3-siTC), and a minimal concentration of 50nM was used in the present study according to transfection efficiency, ×200. (C) The expression of HDAC2 or 3 mRNA in <t>siHDAC2</t> or 3 transfected LX2 cells was detected by qRT-PCR. (D) Knockdown of HDAC2 and <t>HDAC3</t> by siRNAs enhanced the acetylation of Histone H3, detected by western blot using an antibody against acetylated lysine (K) 27 of histone H3 (AcH3K27). (E, F) The expression of VPA-miRNAs (E) and mRNAs of VPA-protein-encoding genes (F) in siHDAC2 and 3 co-transfected LX2 cells was detected by qRT-PCR, all expressions were compared to untreated control or non-targeting siRNA negative control (siNC), *** P < 0.001, ** P< 0.01, *P < 0.05.
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    Image Search Results


    VPA regulated miRNA expression through increased histone acetylation. (A) VPA treatment increased the global protein acetylation of LX2 cells as detected by western blot using an antibody against pan acetyl-lysine, the band for histone was about 15KD. (B) The transfection condition for LX2 cells was optimized by Cy3 labeled siRNA transfection control (Cy3-siTC), and a minimal concentration of 50nM was used in the present study according to transfection efficiency, ×200. (C) The expression of HDAC2 or 3 mRNA in siHDAC2 or 3 transfected LX2 cells was detected by qRT-PCR. (D) Knockdown of HDAC2 and HDAC3 by siRNAs enhanced the acetylation of Histone H3, detected by western blot using an antibody against acetylated lysine (K) 27 of histone H3 (AcH3K27). (E, F) The expression of VPA-miRNAs (E) and mRNAs of VPA-protein-encoding genes (F) in siHDAC2 and 3 co-transfected LX2 cells was detected by qRT-PCR, all expressions were compared to untreated control or non-targeting siRNA negative control (siNC), *** P < 0.001, ** P< 0.01, *P < 0.05.

    Journal: International Journal of Biological Sciences

    Article Title: Crosstalk between Epigenetic Modulations in Valproic Acid Deactivated Hepatic Stellate Cells: An Integrated Protein and miRNA Profiling Study

    doi: 10.7150/ijbs.28642

    Figure Lengend Snippet: VPA regulated miRNA expression through increased histone acetylation. (A) VPA treatment increased the global protein acetylation of LX2 cells as detected by western blot using an antibody against pan acetyl-lysine, the band for histone was about 15KD. (B) The transfection condition for LX2 cells was optimized by Cy3 labeled siRNA transfection control (Cy3-siTC), and a minimal concentration of 50nM was used in the present study according to transfection efficiency, ×200. (C) The expression of HDAC2 or 3 mRNA in siHDAC2 or 3 transfected LX2 cells was detected by qRT-PCR. (D) Knockdown of HDAC2 and HDAC3 by siRNAs enhanced the acetylation of Histone H3, detected by western blot using an antibody against acetylated lysine (K) 27 of histone H3 (AcH3K27). (E, F) The expression of VPA-miRNAs (E) and mRNAs of VPA-protein-encoding genes (F) in siHDAC2 and 3 co-transfected LX2 cells was detected by qRT-PCR, all expressions were compared to untreated control or non-targeting siRNA negative control (siNC), *** P < 0.001, ** P< 0.01, *P < 0.05.

    Article Snippet: LX2 cells were seeded into 6-well culture plates and transfected with small interfering RNAs (siRNAs) against HDAC2 and HDAC3 (siHDAC2, siHDAC3) (RiboBio) using SuperFectin II in vitro DNA transfection reagent (Pufei Biotech, Shanghai, China) according to the manufacturers' instructions.

    Techniques: Expressing, Western Blot, Transfection, Labeling, Control, Concentration Assay, Quantitative RT-PCR, Knockdown, Negative Control